Compatible with chemiluminescent substrates and fluorescent secondary antibodies. Western blotting: detection of the two unstained bands via the detection method used for the target protein. Request sample Interactive selection tool.Mixture of 10 recombinant proteins of precise molecular weights (10250 kD) 8 blue-stained bands and 2 pink reference bands (25 and 75 kD) Features and Benefits. Western blot confirmation-detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots.Fluorescent visualization-detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › Visualize proteins on gels and blots without staining and destaining steps, and use total protein normalization for quantitative western blots. Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2.IBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. 2-DE is an essential step in protein characterization, purification and profiling, and posttranslational modification studies. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed. For colorimetric or chemiluminescence blot detection, StrepTactin-HRP or -AP conjugate is also needed. Protein standards for western blotting applications selection guide. Recombinant Precision Plus Protein Standards. Learn more about the relative transfer efficiencies of tank, semi-dry, and rapid blotting systems for western blotting with large. Bio-Rad's protein standards for western blotting applications. Transfer of High-MolecularWeight Proteins. Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best. Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence ![]() ![]() Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) Visible monitoring of gel separation and transfer efficiency Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins
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